演題抄録

International Session(Poster)

開催概要
開催回
第51回・2013年・京都
 

Isolation of oral squamous cell carcinoma cells with highly lymphatic metastasis

演題番号 : ISP-5

[筆頭演者]
Ya-Wen Chen:1 
[共同演者]
Yi-Chen Yen:1、Shih-Sheng Jiang:1、Wei-Hung Liu:1、Hsiao-Chein Chu:1

1:National Institute of Cancer Research, National Health Research Institutes, Taiwan

 

Oral squamous cell carcinoma (OSCC) is the most prevalent malignant tumor in head and neck region and frequently metastasizes to the cervical lymph nodes, leading to poor survival of patients with OSCC. However, the underlying mechanisms of lymph node metastasis are not fully understood. In this study, the orthotopic transplantation in immune-compromised system was established to feasibly study tumorigenesis and lymph node metastasis of OSCC. Human OSCC cells (OEC-M1) were injected orthotopically into the oral cavity of nude mice. Through in vivo selection, two sublines, designated as LN1-1 and LN1-2 were successfully isolated from cervical lymph nodes. Confirmed by polymerase chain reaction (PCR) with short tandem repeat (STR) amplification, these two sublines were identified to origin from OEC-M1 cells. Importantly, these two sublines metastasized to cervical lymph nodes in 83% of mice, while parental OEC-M1 cells did not metastasis after injection into the oral cavity. However, the parental OEC-M1 cells and sublines did not show the different abilities of in vivo tumorigenesis. OEC-M1, LN1-1 and LN1-2 which have the same origin but different lymphatic metastastic capacities could be a useful model system for study mechanisms involved in lymph node metastasis of human OSCC. We are performing the microarray gene expression analysis to understand the detailed mechanisms.

前へ戻る