演題抄録

International Session(Oral)

開催概要
開催回
第51回・2013年・京都
 

PEPT1 is a valuable biomarker for predicting efficacy of ALA-PDT in PC

演題番号 : ISO1-6

[筆頭演者]
Yang Liu:1 
[共同演者]
Shun-ichiro Ogura:2、Yoshio Endo:3、Emel Canbay :1、Haruaki Ishibashi :1、Yutaka Yonemura :1

1:NPO Organization to Support Peritoneal Dissemination Treatment, Japan、2:Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan、3:Central Research Resource Branch, Cancer Research Institute, Kanazawa University, Japan

 

Objective: 5-aminolevulinic acid based photodynamic therapy (ALA-PDT) has developed into a new important treatment for patients with cancer. It has been studied in a variety of neoplasm and non-neoplasm disease. Peptide transporter (PEPT1) and human ATP-binding cassette (ABC) transporter ABCG2 were found play pivotal roles in the regulation of PpIX level and cellular photo-sensitivity in cancer cells treated with 5-ALA. This study aims to understand the feasibility of application of ALA-PDT in cancer patients with peritoneal dissemination and identify a valuable biomarker for predicting the efficacy of ALA-PDT. Methods: 5-ALA was administrated orally at a dose of 1g/50kg 2 hours before cytoreductive surgery in cancer patients with peritoneal dissemination. During surgery, abdominal cavity was observed under blue-violet light. Normal tissue and cancer tissue were then taken respectively and submitted for both pathological examination and PCR.Results: A total of 11 patients were enrolled in this study. Peritoneal disseminated nodules of 5 patients emitted red fluorescence under blue-violet light, while 5 patients with pseudomyxoma peritonei of appendiceal origin and 1 patient with ovarian cancer were negative for 5-ALA. 5-ALA positive tissues were all invaded with cancer cells. PEPT1 were relatively high expressed in all cancer tissue of 5-ALA positive patients, with a specificity of 100%. However, ABCG2 and PEPT2 were expressed in cancer tissue without specificity.Conclusions: PEPT1 is a valuable biomarker for predicting efficacy of ALA-PDT. Moreover, ALA-PDT could be well applied in patients with peritoneal dissemination of certain tumors.

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