演題抄録

International Session

開催概要
開催回
第55回・2017年・横浜
 

大腸癌における完全長LGR5陽性細胞の意義についての研究

演題番号 : IS6-1

[筆頭演者]
高橋 秀和:1 
[共同演者]
大澤 日出樹:1、西村 潤一:1、原口 直紹:1、畑 泰司:1、山本 浩文:1、松田 宙:1、水島 恒和:1、土岐 祐一郎:1、森 正樹:1

1:大阪大学・大学院医学系研究科・消化器外科学講座

 

Background: Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) is a target of Wnt signaling and considered both a cancer stem cell marker and intestinal stem cell marker. We found firstly some splice variants of LGR5 in human intestine and elucidated the functional feature of full length LGR5 (LGR5FL).
Methods: Reverse transcript polymerase chain reaction using mRNA extracted from intestine revealed the existence of LGR5 splice variants. We designed an antibody that recognizes only LGR5FL and assessed immunohistochemically the distribution of LGR5FL-positive cells and Ki-67-positive cells in clinical samples.
Results: Two LGR5 splice variants were expressed in the human intestine crypt cells; one lacked exon 5 and the other lacked exons 5-8. Only LGR5FL appeared during cell cycle arrest, whereas the transcript variants appeared when the cell cycle was proceeding. Immunohistochemistry and in situ hybridization showed that LGR5FL-positive cells were negative for Ki-67. Comparing pre-chemotherapy and post-chemotherapy specimens, the population of LGR5FL-positive cells significantly increased with therapy (P<0.01).
Conclusion: The function of LGR5FL-positive cells seems to be closer to that of cancer stem cells than its splice variants, and designing therapeutic strategies that target LGR5FL-positive cells seems to be important in colorectal cancer.

キーワード

臓器別:大腸・小腸

手法別:基礎腫瘍学

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