演題抄録

一般演題 (示説)

開催概要
開催回
第54回・2016年・横浜
 

The expression and function of L1CAM in cervical cancer--promotes tumor progression and is an independent unfavorable prognostic factor

演題番号 : P25-3

[筆頭演者]
Jue Zhang:1 
[共同演者]
Xufeng Wu:1

1:Hubei Maternal and Child hospital, China・Gynecologic Oncology

 

To explored the expression of L1CAM in cervical cancer tissues and analyzed its clinical significance. The function of L1CAM in the progression of cervical cancer was also addressed.Firstly, L1CAM was over-expressed in cervical cancer by comparing to the normal epithelium. In cervical cancer tissues, L1CAM are distributed in cell membrane, cytoplasm and/or the nucleus, showing the characteristics of its diversity in expression styles. The expression of L1CAM in cell nucleus was significantly associated with the differentiation (P = 0.02) of Hela cells.Over-expression of L1CAM was associated with poor survival of cervical cancer patient (membrane/cytoplasm expression: P = 0.018; nuclear expression: P = 0.034; membrane/cytoplasm and nuclear expression: P = 0.041).Secondly, L1CAM was highly espressed in most of the cell lines detected and was mainly distributed in the membrane and nucleus of cervical cancer cells. In certain cells lines, L1CAM was negative or low-expressed. The immunofluorescence staining showed that inhibitors of ADAMs and γ-secretase inhibited the nuclear translocation of L1CAM.Thirdly, RNAi-mediated knockdown of LICAM decreased the proliferation, migration and invasion of cervical cancer cells while over-expression of L1CAM did the opposite.Finally, Data suggested that 3402 genes were differentially expressed upon L1CAM knockdown (2456 genes were up-regulation and 946 genes were down-regulation). Functional enrichment analysis showed that some proliferation-related genes or invasiveness-related genes were changed in the RNAi-treated cells, indicating that L1CAM might affect the proliferation and invasiveness by altering the expressions of these genes. Further analysis showed that, besides the signalling pathways described above, the ubiquitin mediated proteolysis pathway, PI3K/Akt pathway, mTOR pathway and cell cycle pathway were also changed upon L1CAM knockdown.

キーワード

臓器別:子宮

手法別:トランスレーショナルリサーチ

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