演題抄録

一般演題 (示説)

開催概要
開催回
第53回・2015年・京都
 

骨巨細胞腫におけるデノスマブ治療後の網羅的タンパク質発現解析

演題番号 : P146-2

[筆頭演者]
向井原 健太:1 
[共同演者]
末原 義之:1、窪田 大介:1、石井 翠:1、赤池 慶祐:1、金子 和夫:1、斎藤 剛:2

1:順天堂大学医学部整形外科、2:順天堂大学医学部人体病理

 

Introduction: Giant cell tumor of bone (GCTB) is a locally aggressive osteolytic bone tumor. Recently, some clinical trials have shown that a monoclonal RANKL antibody, denosumab, is a novel and effective therapeutic option for aggressive and recurrent GCTB. However, the protein expression profiles and functional networks of GCTB treated with denosumab are still unknown. In this study, to understand the protein expression of GCTB treated with denosumab, we conducted proteomic studies using clinical samples obtained both pre- and post-treatment with denosumab.
Methods: To identify the protein profiles of GCTB associated with denosumab treatment, we conducted proteomic analyses using two pairs of pre- and post-denosumab treatment samples. We employed i-TRAQ to identify the protein profiles. In order to further understand the affected biological networks, we applied the Ingenuity Pathways Analysis (IPA) system using each protein profile.
Results: Proteins extracted from the pairs were analyzed using the i-TRAQ method. The analyses identified approximately 1,600-2,200 proteins in each sample. We analyzed the two pairs of profiles to identify proteins that were similarly altered in both sample sets, and found 37 consistently upregulated and 32 consistently downregulated proteins. In the network analyses using IPA, we performed network assessments using each protein profile and common protein profiles. In these independent analyses using each data set, the network assessments identified several key proteins whose upstream regulators play a critical functional role in the RANKL pathway.
Discussion: This data is the first report regarding the proteomic changes of GCTB treated with denosumab. We successfully identified several proteins associated with denosumab treatment in GCTB samples. We believe that the identified proteins and the results of the network analysis will help to understand the effects of denosumab treatment on GCTB.

キーワード

臓器別:骨軟部

手法別:分子標的治療

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