演題抄録

FACO International Workshop

開催概要
開催回
第53回・2015年・京都
 

Epigenetic silencing of miR-200 family is associated with regulation of ZEB1/ZEB2 in gastric cancer metastasis

演題番号 : FWS8-2

[筆頭演者]
Kim Dong Yi:1 
[共同演者]
Kim Ho Gun:1、Ryu Seong Yeob:1、Lee Jae Hyuk:1

1:Gastroenterologic Surgery, Department of Surgery, Chonnam National University Medical School

 

Background: Epigenetic silencing of miRNAs with tumor suppressor activity by CpG island methylation plays an important role in the development and progression of gastric cancer. Materials and Methods: The extent of promoter methylation of the miR200-ba429, miR200-c141 was assessed qualitatively using methylation-specific PCR (MSP) in 122 gastric cancers staged from I to IV. The immunohistochemical assessment of ZEB1, ZEB2, E-cadherin, and MUC-1 gene expression was done in gastric cancer tissues. Results: The methylation frequency for the miR200-ba429, miR200-c141, and E-cadherin gene according to MSP techniques was significantly higher in AGCs than in EGCs (88% and 68%, 83% and 52%, 64% and 52%, P<0.05). Significant positive correlation of miR200-ba429, miR200-c141 hypermethylation with ZEB1/ZEB2 and MUC1 expression was identified in gastric cancer. Methylation status of miRNA 200 family as well as expression of ZEB1/2, E-cadherin, and MUC1 was significantly correlated with tumor differentiation and clinical stages (P<0.05). Conclusion: The ZEB1/2-miRNA 200 family feedback loop might be a promising molecular target for gastric carcinogenesis and metastasis.

キーワード

臓器別:胃・十二指腸

手法別:トランスレーショナルリサーチ

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