FACO International Workshop
Establishment of novel predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis
演題番号 : FWS3-1
1:Gastroenterological and Pediatric Surgery, Faculty of Medicine, Oita University、2:Colon and Rectal Surgery, Weill Cornell Medical College
Improving recurrence prediction for resected colorectal cancer patients could modify surveillance strategies, providing opportunities for more informed use of chemotherapy for high risk individuals. Changes in the glycosylation patterns on cells and specific glycotransferases are reportedly related to carcinogenesis, tissue adhesion, and metastasis. Recently, a novel lectin microarray system was developed to comprehensively analyze glycan profiles using lectins that specifically recognized various sugar moieties. The aim of this study was to identify predictive factor for distant recurrence of colorectal cancer using a novel lectin microarray system.
Glycoproteins were extracted from formalin-fixed, paraffin-embedded tumor specimens and normal epithelium from 53 consecutive curatively resected stage I-III colorectal cancer cases. Samples were subjected to lectin microarray to obtain lectin-glycan interaction (LGI) values. Then, clinicopathologic factors including LGI values that associated with distant recurrence were identified. The results were further validated by additional dataset with 55 curatively resected stage II colorectal cancer patients.
LGI values for Agaricus bisporus lectin (ABA) and Hippeastrum hybrid lectin (HHL) were statistically associated with distant recurrence in a multivariate analysis. In the validation set, ABA-binding signals were independent predictive factors for distant recurrence. Kaplan-Meier survival curves demonstrated the patients with low ABA- binding signals showed better distant specific recurrence- free survival compared to those with high ABA- binding. In histochemical analysis, ABA lectin staining exhibited strikingly intense signals in the cytoplasm and apical surfaces of cancer cells, while weak staining was observed in the supranuclear regions of normal epithelium.
ABA tumor/normal LGI ratio could be a novel predictive biomarker for distant recurrence of curatively resected colorectal cancer.