演題抄録

International Symposium

開催概要
開催回
第52回・2014年・横浜
 

Analyses of mechanism of epithelial tissue homeostasis in the gastrointestinal tract, and dysregulated maintenance during cancer development by the multicolor lineage tracing method

演題番号 : TIS2-4

[筆頭演者]
Ueno Hiroo:1 

1:Department of Stem Cell Pathology, Kansai Medical University

 

 Generation of chimeras, which is now a standard technology for producing gene modified mutant mice, was originally developed as a tool for developmental biology. However, application of conventional single marker chimeric mice for developmental study had been limited. This situation has been dramatically changed by development of multicolor chimeric mice using various kinds of fluorescent proteins. Now by our technology (the cre-loxp-mediated multicolor mosaic mice; rainbow mice), up to ten different clones could be distinguished by their colors, which enable us to perform more accurate statistical analyses and lineage tracing experiments than conventional methods. The method could be applied to visualize not only cell turnover of normal stem cells but also cancer development of live tissues in vivo.
 Since the discovery of intestinal stem cells (ISCs) in the small intestine, vast amount of knowledge has been accumulated regarding the mechanism of maintenance of gastrointestinal epithelial tissues. However, many questions have remained to be elucidated. One important question is clonality of origin of intestinal tumors. Generally, the origin out malignant tumors is considered to be monoclonal. However, benign adenomas in the familial adenomatous polyposis (FAP) patient or in FAP model mice, Apcmin, their origin of tumors has been reported to be polyclonal. Another important question would be the lineage relationship between normal and cancer stem cells in the tissues.
 Moreover, stem cells in the upper gastrointestinal tract including esophagus and tongue epithelia have been unidentified, and therefore the origin of malignant tumors in the tissues have been largely ununderstood. We very recently identified lingual epithelial stem cells by using our methodology.
 In this presentation, application of our multicolor lineage tracing method to issues raised above will be discussed.

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